DNA Polymerase Flashcards
DNA Polymerase catalyzes the formation of …
A phosphodiester bond
Where does a phosphodiester bond form?
Between the 3’ OH group of Deoxyribose and 5’ phosphate of dNTP precursor
What are the two purposes of DNA polymerase III?
1)Phosphodiester Bond formation(elongation)
2) Repair of base misincorporation
What is released at formation of phosphodiester bond?
Pyrophosphate
How is base misincorporation repaired by DNA Polymerase ?
3’ to 5’ proofreading exonuclease activity of DNA Polymerase clips off unpaired residues at primer terminus.
Where does DNA add nucleotides ?
To a free 3’ end of a growing strand
What does primase do in DNA synthesis?
It is an enzyme that can start an RNA chain from scratch and add RNA nucleotides (Uracil vs Thymine) one at a time.
Where does primase begin synthesis?
3’ end of the DNA
Function of DNA Polymerase I
Removes and replaces RNA primer
