DIFFERENTIAL COUNT Flashcards
______: most commonly used method
- aka
- after moving towards the center, move two fields _____ and back to the side edge _____ times
- purpose: to compensate on the inconsistency distribution of cells since most of the ______ are located at the edge while the ____ are located at the body/center
BATTLEMENT METHOD
- Serpentine
- VERTICALLY, 2
- LARGE/NUCLEATED CELLS
- SMALLER (e.g. LYMPHOCYTES)
_____: move the fields from end to end and go back from the same end to the other end
- ideal only when the smear is too ___ enough
- with ____, this is not possible as cells are too much overlapping and distorted
- examination are only limited to the ___ of the smear
- can be done if the smear is ____
- prepared with the use of ___
LONGITUDINAL STRIP METHOD
- THIN
- WEDGE SMEAR
- CENTER
- SPUN SMEAR
- HEMASPINNER
____: pass from the edge or the side and then the center to compensate the distribution of cell
____: WBCs are counted in consecutive fields as the blood film is moved from side to side
- aka
STEP-METHOD
CROSS-SECTIONAL
- Crenellation Method
PROCEDURE
- scan to review blood film
- Check feather edge for
- Check film edge for
- Edges should contain ____ more WBCs than in the body of the film
- rbc should be color = ___; platelets should be color = ____
- scan to determine examination area
- Atleast___oftheRBCsshouldnotbe overlapping - scan to PerformWBCdifferentialcount
- LPO
- FIBRIN
- EXCESSIVE LEUKOCYTES
- <2-3
- SALMON PINK; PURPLE/BLUISH
- HPO
- 50% - OIO
The more cells there are, the WBCs should also be counted.
- 50 cells: wbc count is < ______/L or > _____/uL
- 200 cells: wbc count is > ______/L or > _____;uL
- more wbc should be counted: _____/uL
- <1x10^9/L or >1000/uL
- > 40x10^9/L or >40000/uL
- > 100,000/uL
If the count is less than 100, we _____ the count by a factor to make it a total of 100
If we counted more than 100, we have to report it based on ____ totality. In this case, we _____ the cells in a factor to convert it to a total of 100.
multiply
200; divide
How to estimate platelets?
has to be at least _____ platelets/ oil immersion field (oif) wherein each of this oif, there are approximately ____ RBCs.
8-20 platelets / oil immersion field
200 rbcs
TWO COUNTER
- Cannot be used in differential counter because it will not show the types of WBCs
- used for ____ or _____ - Counts and identify the cells separately
- TALLY COUNTER
- total cell counting or total
enumeration - DIFFERENTIAL COUNTER
Additional 100 cells are counted when:
- ___ eosinophils
- ___ basophils
- ___ monocytes
- ____ > neutrophils
- it is valid if the lymphocytic observation is observed if the blood sample is from ______
- > 10% eosinophils
- > 2% basophils
- 11% monocytes
- lymphocytes > neutrophils
- children
METHODS OF CLASSIFYING NEUTROPHILS
______: Classification based on age and the age is dependent on the number of lobes or segments
- % distribution
- class I
- class II
- class III
- class IV
- class IV
ARNETH’S CLASSIFICATION
- % distribution
- 5%
- 35%
- 41%
- 17%
- 2%
METHODS OF CLASSIFYING NEUTROPHILS
______: dependent on the granulations
- less granules = ____
- makes use the ____ to tally the cells
- __ shift: indicates younger forms
- __ shift: indicates older forms
- Normal blood sample: shows a ___ Shift
SCHILLING’S CLASSIFICATION
- immature
- Schilling’s Hemogram
- Left
- Right
- Right
SCHILLING’S HEMOGRAM
Shift to Left
1. _____: ⬆️ young forms with accompanying ⬆️ wbc colors count
- cause (3)
2. _____: ⬆️ young forms with normal or ⬇️ wbc colors count
- cause (2)
Shift to Right
1. diseases (2) due to RBCs and Neutrophils will appear abnormal.
Shift to Left
1. Regenerative
- Infection,appendicitis and acute sepsis
2. Degenerative
- TyphoidFeverandTB
Shift to Right
1. Pernicious anemia or megaloblastic anemia
METHODS OF CLASSIFYING NEUTROPHILS
______: presence of filaments that connects the lobes or the segments of the nucleus.
- mature; immature
FILAMENTOUS AND NON-FILAMENTOUS
- Filamentous; Non-Filamentous
