DEHYDRATION

Question 1

Factors To Be Considered

Volume
1. ___ the volume of tissue

Prolonged Immersion
1. high concentrations: tissues become ____
2. low concentrations: tissues become ____

Temperature:
1. ____

____: ¼ inch at the bottom of the container to
facilitate the removal of water molecules from the dehydrating fluid

Answer 1

Volume
1. 10x

Prolonged Immersion
1. hard and brittle
2. mcerated

Temperature:
1. 37C

Anhydrous Copper Sulfate

Question 2

ALCOHOL DEHYDRANTS

1. best dehydrating agent, fast-acting and miscible in water and many organic solvents
2. substitute for ethanol
3. for blood & tissue films and smear preparations
   - aka

Answer 2

1. ETHANOL
2. ISOPROPYL ALCOHOL
3. METHANOL
   - WOOD ALCOHOL

Question 3

OTHER DEHYDRANTS
1. cheap, rapid and used for most urgent biopsies
2. rapid and does not cause any harmful effect on tissues; toxic to reproductive, fetal, urinary & blood systems
- aka
- combustible at
- ____ -based glycol ethers may be used in place of EGME
3. may be used to dehydrate sections and smears after staining

Answer 3

1. ACETONE
2. CELLOSOLVE
   - Ethylene Glycol Monoethyl Ether
   - 110-120F
   - PROPYLEME -based
3. TRIETHYL PO4

Question 4

OTHER DEHYDRANTS
1. expensive and extremely dangerous; vapor tends to accumulate in the body and is highly toxic; creates explosive peroxides
- aka
- uses several changes of pure dioxane
- uses pure dioxane & anhydrous calcium
oxide or quicklime to facilitate removal of water

2. vapors cause nausea, dizziness, headache and anesthesia; skin
   and eye irritant; offensive odor
   - may cause

Answer 4

1. Dioxane
   - Diethylene Dioxide
   - Graupner’s Method
   - Weiseberger’s Method
2. Tetrahydrofuran
   - conjunctivitis

Question 5

Additives to Dehydrants
1. added to 95% alcohol to soften tissues
2. used to soften hard tissues (2)

Answer 5

1. 4% PHENOL
2. Glycerol-alcohol mixture & Molliflex