D1.1 DNA replication SL Flashcards

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1
Q

DNA Replication

A

Making exact DNA copies for reproduction and growth

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2
Q

Semi-Conservative Replication

A

Combines one old and one new DNA strand

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3
Q

Helicase Function

A

Unzips DNA double helix

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4
Q

DNA Polymerase Function

A

Adds nucleotides to form DNA strand

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5
Q

PCR Purpose

A

Amplifies small DNA fragments

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6
Q

Gel Electrophoresis Use

A

Separates DNA by size, charge

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7
Q

PCR Application

A

Forensic DNA profiling

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8
Q

PCR Process

A

Heat DNA, anneal primers, replicate

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9
Q

PCR Amplification

A

Doubles DNA each cycle

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10
Q

DNA Fingerprinting

A

Unique DNA profiles via electrophoresis

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11
Q

DNA Ladder

A

Size reference in electrophoresis

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12
Q

PCR Primer

A

Initiates DNA replication

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13
Q

Taq Polymerase

A

Heat-stable for PCR

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14
Q

Annealing Phase in PCR

A

Primers bind to DNA

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15
Q

Extension Phase in PCR

A

Taq polymerase extends DNA

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16
Q

Denaturation Phase in PCR

A

Heat separates DNA strands

17
Q

Forensic DNA Profiling

A

Identifies individuals from DNA