D1.1 DNA replication SL Flashcards
DNA Replication
Making exact DNA copies for reproduction and growth
Semi-Conservative Replication
Combines one old and one new DNA strand
Helicase Function
Unzips DNA double helix
DNA Polymerase Function
Adds nucleotides to form DNA strand
PCR Purpose
Amplifies small DNA fragments
Gel Electrophoresis Use
Separates DNA by size, charge
PCR Application
Forensic DNA profiling
PCR Process
Heat DNA, anneal primers, replicate
PCR Amplification
Doubles DNA each cycle
DNA Fingerprinting
Unique DNA profiles via electrophoresis
DNA Ladder
Size reference in electrophoresis
PCR Primer
Initiates DNA replication
Taq Polymerase
Heat-stable for PCR
Annealing Phase in PCR
Primers bind to DNA
Extension Phase in PCR
Taq polymerase extends DNA