CHEMICAL EXAM

Question 1

ALBUMIN QUALITATIVE:

Answer 1

HEAT AND ACETIC ACID - first morning urine specimen
SSA/EXTON - SSA/sulfosalicylic acid
HELLER TEST- concentrated nitric acid
ROBERT - magnesium sulfate and nitric acid

Question 2

ALBUMIN QUANTITATIVE

Answer 2

1. KWILECKI’s MODIFICATION OF ESBACH’S METHOD - 24 HOUR URINE SPECIMEN
   - picric acid and citric acid
2. KINGSBURY AND CLARK METHOD - 3% SULFOSALICYLIC ACID

Question 3

GLUCOSE QUALITATIVE

Answer 3

FRESHLY VOIDED OR RANDOM SPECIMEN
1. BENEDICTS TEST- CSS (Copper sulfate, sodium carbinate, sodium citrate)
2. FEHLING’S TEST - A( cupric sulfate)
B(rochelle’s salt and KOH)
3. NYLANDER TEST- rochelleks salt, 10% KOH, BISMUTH SUBNITRATE
4. MOORE-HELLER TEST - 10% KOH

Question 4

FRUCTOSE

Answer 4

FIRST MORNING SPECIMEN
1. SELIWANOFF - resorcinol, HCL
2. BORCHARDT 25% HCL with resorcinol crystals
3. RUBNER’S - LEAD ACETATE

Question 5

PENTOSES

Answer 5

BIAL ORCINOL

ORCINOL
HCL
FERRIC CHLORIDE

Question 6

FIRST MORNING SPECIMEN

Answer 6

1. heat and acetic acid ni albumin quali
2. seliwanoff ni fructose, borchardt, rubner
3. bial orcinol ni pentose

Question 7

FRESHLY VOIDED OR RANDOM SPECIMEN

Answer 7

1. exton, heller, robert ni albumin quali
2. benedict and fehling, nylander and moore heller ni glucose

Question 8

24 hour urine specimen

Answer 8

albumin quantitative:
esbach’s method
kingsbury and clarks method