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Phlebotomy > Chapter 10 Blood Collection Procedures > Flashcards

Chapter 10 Blood Collection Procedures Flashcards

1
Q

Plasma

A

Liquid portion of the UNCLOTTED blood and still contains clotting factors

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2
Q

Serum

A

liquid portion of blood that has been allowed to clot (coagulate). Clotting factors are no longer present as they have been used to clot the blood.

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3
Q

Tubes with anticoagulants

A

Light blue
Green (light or dark)
Pink
Lavender
Royal blue with a lavender stripe
Gray

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4
Q

What will tubes with anticoagulants produce?

A

Plasma when separated in a centrifuge

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5
Q

Tubes without anticoagulants List (4)

A

Clear-used as a discard tube
Red-glass
Tiger Top/Gold Top/ Marble Top/ SST
Royal blue with red stripe

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6
Q

What will tubes without anticoagulants produce?

A

Serum when separated in a centrifuge

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7
Q

What is the order of draw

A
  1. Blood Culture Bottles (Broth) BCX2
  2. Clear or Red (glass)/Light blue top (Sodium Citrate) 100% full
  3. Royal Blue with Red Stripe (none)
  4. Red top (Clot Activator if plastic)
  5. Gold, Tiger, Marble, SST (Silica/Gel)
  6. Light green top (Lithium Heparin/Gel) (PST)
  7. Dark Green Top (Sodium Heparin) *put on ice
  8. Pink Top (Potassium EDTA) 2/3 full
  9. Lavender top (EDTA)
  10. Royal Blue w/Lavender Stripe (EDTA)
  11. Gray Top (Sodium Fluoride / Potassium Oxalate)

Dark Blue (As called for in draw order)

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8
Q
  1. Blood Cultures
A

x2
Additive-Nutrient Broth
Lab responsible for specimen testing: Microbiology

Each set consists of two bottles
Aerobic (w/oxygen)
Anaerobic (w/out oxygen)

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9
Q
  1. Clear Top Tube or Red top tube
A

Discard to avoid Thrombin and other cells

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10
Q
  1. Light Blue Top Tube
A

Additive: Sodium Citrate
Yields: Plasma
Lab responsible for specimen testing: Coagulation or Hematology

9 parts blood to 1 part additive (filled completely)
3-4 times inverted

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11
Q

Common Tests for Light Blue

A

Prothrombin Time (PT)-
Partial Thromboplastin Time (PTT)-
Activated Partial Thromboplastin Time (aPTT)

D-Dimer

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12
Q
  1. Red Top Tube Clot
A

Additive: Glass-none
Plastic-clot activator (S)ETOH
Yields: Serum

Lab responsible for specimen testing:Chemistry

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13
Q

Common Tests for Red Top Tube

A

“Plan vacuum tube” no addditve or anticoagulant. In this tube, blood will clot naturally within 30-60 minutes

serum hCG
serum ETOH

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14
Q
  1. Tiger/Marble/Red-Gray Speckled, Gold or SST Top Tube additives
A

Additive: Silica (clot activator) & Thixotropic Gel (serum separator)
Yields: Serum

Lab responsible for specimen testing: Chemistry

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15
Q

Common Tests for SST Tube

A

Basic Metabolic Panel (BMP)
Complete/Comprehensive Metabolic Panel/Profile (CMP)
Serum hCG
Lipids/Cholesterol
Hepatic Panel
Progesterone

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16
Q
  1. Light (Lithium Heparin) Green Top Tube (PST)
    Additives
A

Additive: Lithium Heparin (an anticoagulant), and Thixotropic Gel (a plasma separator)
Yields: Plasma

Lab responsible for specimen testing: Chemistry

17
Q

Common Tests for Light PST (LITHIUM HEPARIN & THIXOTROPIC) green top tube

A

STAT Electrolytes “LYTES”-

(P) hCG

(P) hCG qualitative-

(P) hCG quantitative

18
Q
  1. Dark Green Top Tube (Sodium Heparin)
A

Additive: Sodium Heparin an anticoagulant
Yields: Plasma

Lab responsible for specimen testing: Chemistry

19
Q

Common tests for Dark Green Top Tube

A

Ammonia

**Must be placed on ice

20
Q
  1. Pink Top Tube (P) in (K2)
    Additives
A

Additive: Potassium EDTA
Yields: Plasma

Lab responsible for specimen testing: Blood Bank

2/3 full or more

21
Q

Common Tests for Pink Top Tube

A

Type and Screen Ratio (TSR)

Cross Match

22
Q
  1. Lavender or Light/Dark Purple Top Tubes
    Additives
A

Additive: Ethylendiaminetetraacetic Acid (EDTA)
Yields: Plasma

Lab responsible for specimen testing: Hematology

23
Q

Common tests for Lavender or Light/Dark Purple Top Tubes (7)

A

Erythrocyte Sedimentation Rate (ESR)
Sickle Cell
Complete Blood Count (CBC) **never centrifuged
Hematocrit
Hemoglobin
Platelets
Hemoglobin A1C (HgA1C)- 3 month span for diabetes

24
Q
  1. Gray Top Tube
    Additives
A

Additives: Sodium Fluoride an antiglycolytic agent (glucose preservative) and Potassium Oxalate an anticoagulant
Yields: Plasma

Lab responsible for specimen testing: Chemistry

25
Q

Common Tests for Gray Top Tube (3)

A

Oral Glucose Tolerance Test (OGTT)

Lactic Acid
Should be drawn without stasis (no tourniquet applied)
**sample must be placed on ice

Alcohol (P) ETOH- do NOT clean the site with alcohol

26
Q

Sodium Fluoride

A

Preservative that prevents the breakdown of sugar. It will preserve the integrity of glucose in the sample for 3 days

27
Q

If glucose test requires serum which tube top color should be used instead of Gray?

A

SST

28
Q

Potassium Oxalate

A

Is an anticoagulant that prevents clotting by binding to calcium in the sample.

29
Q

**Royal Blue Top Tube
Both additives

A

Additive: Red Stripe-None
Blue/Lavender Stripe-EDTA

Yields: Red Stripe-Serum
Blue/Lavender Stripe-Plasma

Lab responsible for specimen testing: Toxicology

30
Q

**common Test for Royal Blue Top Tube

A

Lead (Pb) Poisoning
Heavy Metal Toxicology

31
Q
  1. Royal Blue Top Tube with Red Stripe
    No additive tests
A

Lead (Pb) Poisoning
Heavy Metal Toxicology

32
Q
  1. Royal Blue Top Tube with Blue/Lavender Stripe
    EDTA
    Tests
A

Lead (Pb) Poisoning
Heavy Metal Toxicology

33
Q

What does the Royal Blue Top Tube with Red Stripe yield?

A

Serum

34
Q

What does Royal Blue Top Tube with Blue/Lavender stripe yield

A

Plasma

35
Q

TJC The Joint Commission

A

Solely look out for patients
Establish standards and practices of ops in all healthcare settings
Improve healthcare

36
Q

CLSI Clinical & Laboratory Standards Institute

A

Develops standards
Sets standards globally nfp
Phlebotomy programs approval, cert exam, questions and standard of care based on CLSI standards

Phlebotomy (2 decks)

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