Chapter 1: Microscopy

Question 1

Two advantages of using an electron microscope over a light microscope

Answer 1

1. Higher resolution images
2. Large depth of field - more specimen in focus

Question 2

What is the main use of a light microscope?

Answer 2

Allows us to observe the structures of cells in detail

Question 3

What is the main use of a transmission electron microscope?

Answer 3

Uses a beam of electrons to produce high resolution images of biological samples

Question 4

What is the main use of a scanning electron microscope?

Answer 4

Investigations into nanoscience - can produce 3D images of specimen

Question 5

What is the main use of a laser scanning confocal microscope?

Answer 5

Scans layers of an object to remove background light (higher resolution than light microscope)

Question 6

Define magnification

Answer 6

The increase in size of an object using an optical device

Question 7

Define resolution

Answer 7

Measure of the microscopes ability to distinguish between two points which are close together on an object