Cells Flashcards

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1
Q

The cell-surface (plasma) membrane

A

Mainly made of lipids and proteins
It regulates the movement of substances into and out of the cell
It has receptors on its surface which allows it to respond to chemicals e.g. hormones

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2
Q

The nucleus

A

Surrounded by a nuclear envelope (a double membrane) which has many nuclear pores
Contains chromosomes made from protein-bound linear DNA and one ore more nucleolus structures
It control’s the cells activities by controlling the transcription of DNA
The nucleolus is made up of ribosomes and proteins and makes ribosomes
Contains the hereditary genome

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3
Q

Mitochondrion

A

Double membrane
The inner membrane us folded into structures called cristae
Inside is the matrix which contains enzymes involved in respiration
The site of aerobic respiration where ATP is produced

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4
Q

Chloroplast

A

A flattened structure found in plant and algal cells
Double membrane
Thylakoid membranes inside which are stacked up to form grana
Grana are linked together by lamellae (thin, flat pieces of thylakoid membrane)

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5
Q

The Golgi Apparatus

A

A group of fluid-filled membrane-bound flattened sacs
Vesicles often line the edges of the sacs
It processes and packages new lipids and proteins
It makes lysosomes

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6
Q

A Golgi Vesicle

A

A small fluid-filled sac in the cytoplasm which is surrounded by a membrane and produced by the Golgi apparatus
They store lipids and proteins made by the Golgi Apparatus and transports them out of the cell via the cell-surface membrane

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7
Q

A lysosome

A

A round organelle surrounded by a membrane with no clear internal structure
It’s a type of Golgi vesicle that contains digestive enzymes called lysozymes
These are kept separate from the cytoplasm by the surrounding membrane and digest invading cells and break down worn components of a cell

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8
Q

Ribosomes

A

A very small organelle that either floats free in the cytoplasm or is attached to the RER
It’s made up of proteins and RNA
It’s not surrounded by a membrane
Folding and processing proteins
Synthesise proteins

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9
Q

The rough endoplasmic reticulum

A

A system of membranes enclosing a fluid filled space
The surface is covered with ribosomes
It folds and processes proteins that have been made by ribosomes

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10
Q

The smooth endoplasmic reticulum

A

A system of membranes enclosing a fluid filled space
No ribosomes on surface
Synthesizes and processes lipids

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11
Q

The cell wall

A

A rigid structure that supports cells and prevents them changing shape
In plants and algae it’s mainly made of the carbohydrate cellulose
In fungi it’s made of chitin

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12
Q

Cell vacuole

A

A membrane bound organelle found in the cytoplasm of plant cells
It contains cell sap which is a weak solution of sugars and salts
The surrounding membrane is called the tonoplast
Helps maintain pressure inside the cell and keep the cell rigid
Stops the plant wilting
Involved in the isolation of unwanted chemicals

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13
Q

Algal and fungal cells

A

Algal cells have the same organelles as plant cells
Fungal cell walls are made of chitin, not cellulose and they don’t have chloroplasts as they don’t photosynthesise

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14
Q

Cell specialization

A

In multicellular, eukaryotic organisms, specialized cells are grouped together to form tissues
A tissue is a group of cells that work together to form a particular function
Different tissues work together to form organs. Different organs make up organ systems

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15
Q

Prokaryotic DNA

A

No nucleus
DNA floats free in the cytoplasm
Circular DNA
Not associated with histones
Plasmids are small loops of DNA that aren’t part of the main DNA molecule
They contain genes for things like antibiotic resistance and can be passed between prokaryotes. They’re not always present

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16
Q

Prokaryotic cell wall

A

Made from murein
Murein is a glycoprotein - a protein with a carbohydrate attached

17
Q

Flagellum/flagella

A

A long, hair-like structure that rotates to aid the cells movement
Not all prokaryotes have one and some may have several

18
Q

Viruses

A

Acellular (not cells)
Nucleic acids surrounded by protein
Not alive
Smaller than bacteria
Viruses invade and reproduce inside host cells

19
Q

Prokaryotic replication (binary fission)

A

The circular DNA and plasmids replicate
Plasmids can replicate numerous times but the circular DNA only once
The cell gets bigger and the loops of DNA move to opposite poles of the cell
The cytoplasm begins to divide and new cell walls begin to form
The cytoplasm divides and two daughter cells are produced. Each only has one copy of circular DNA but can have a variable number of plasmids

20
Q

Viral replication

A

Viruses use their attachment proteins to bind to complementary receptor proteins on the cell surface membrane of the host cell
Viruses have different attachment proteins therefore require different receptor proteins so some can only attach to one while others can infect lots
They inject their viral RNA into the host cell
RNA interstates with the cells DNA
Reverse transcriptase turns RNA to DNA
Viral proteins are made and replicated

21
Q

Magnification =

A

size of image / actual size

22
Q

What is resolution?

A

How detailed the image is
How well a microscope can distinguish between two points that are close together
If a microscope has low resolution, increasing magnification won’t help

23
Q

Optical (light) microscopes

A

Use light to form a coloured image
Maximum resolution of 0.2 micrometers (um)
Maximum magnification x1500
Organelles smaller than 0.2 um can’t be viewed, this includes ribosomes, endoplasmic reticulum and lysosomes. Mitochondria may be undetailed

24
Q

Electron microscopes

A

Use electrons to form an image
Have a higher resolution than light microscopes so give a more detailed image
Max resolution of 0.0002 um
x 1,500,000

25
Q

Transmission electron microscopes

A

Uses electromagnets to focus a beam of electrons which is transmitted through the specimen
Denser parts absorb more electrons so appear darker on the image
Can only be used on thin specimens
High resolution so can see the internal structures of organelles
Can’t be used to observe live specimens
Black and white image

26
Q

Scanning electron microscopes

A

Scan a beam of electrons across a specimen. Electrons bounce of the surface and form an image
3D image
Thick specimens
Lower resolution than TEMs
Black and white image

27
Q

How to prepare a specimen slide

A

Cut 1cm from the tip of a growing root as that’s where growth occurs
Prepare a boiling tube containing 1M HCl and place in water bath at 60c
Incubate root tip in tube for 5 minutes
Use a pippete to rinse root tip in cold water and leave to dry on a paper towl
Use a mounted needle to break the tip open and thinly spread the cells
Use pipette to add a drop of water onto the slide
Obtain thin section of plant tissue and place on the slide using tweezers
Stain with iodine in potassium iodide
Lower cover slip using mounted needle
Avoid air bubbles. Push down firmly. Do not smear otherwise chromosomes will be damaged - creates a single thin layer of cells so that light can pass through
Artefacts e.g. dust, fingerprints, air bubbles cause inaccuracies
They’re common in electron micrographs as specimens need lots of preparation before viewing under a microscope

28
Q

Cell fractionation methods - homogenization (1)

A

Breaking apart the plasma membrane to release organelles
Buffer solution maintains pH
Ice-cold to reduce enzyme activity
Isotonic same concentration of chemicals to prevent damage to organelles via osmosis

29
Q

Filtration (2)

A

Homogenised cell solution is filtered through a gauze to separate large cell or tissue debris

30
Q

Ultracentrifugation

A

Separates the organelles
Pour cell fragments into a tube and place into a centrifuge and spin at low speed
Heaviest organelles sink to the bottom and form a thick sediment pellet
The lighter organelles remain suspended in the supernatant fluid above the sediment
The supernatant is drained off and spun again at a higher speed
The process is repeated until all organelles are separated out

31
Q

The cell cycle

A

Interphase - Synthesis - DNA replicates
Interphase - Gap phase 2 - Cell continues growing and proteins needed for cell division are made
Mitosis - produces two genetically identical daughter cells. Needed for growth and repair
Interphase - Gap phase 1 - Cell grows and new organelles and proteins are made. ATP content increases

32
Q

Stages of mitosis (PMAT)

A

Prophase - Chromosomes condense and become visible. Centrioles begin to move to opposite ends of the cell forming the spindle fibers. The nuclear envelope breaks down

Metaphase - The chromosomes line up along the middle of the cell and attach to spindle fibres by the centromere

Anaphase - The centromeres divide and the spindle fibres pull each chromatid to opposite poles

Telophase - Chromatids uncoil and become chromosomes. A nuclear envelope forms around each group of chromosomes so there are now two nuclei. Division of the cytoplasm (cytokinesis starts in anaphase)

33
Q

Calculating time taken for each stage of mitosis

A

E.g. a scientist counts 100 cells undergoing mitosis, of which 10 are in metaphase. One complete cell cycle of the tissue takes 15 hours. How long do the cells spend in metaphase. Answer in minutes
15x60 = 900 minutes
(10/100) x 900 = 90 minutes

34
Q

Some cancer treatments target the cell cycle

A

G1 - cell growth and protein production
Chemical drugs like chemotherapy prevent the synthesis of enzymes needed for DNA replication and the cell undergoes apoptosis/ cell death
S phase (DNA replication) Radiation and some drugs damage DNA. Several times during the cell cycle DNA is checked for damage. If severe damage is detected the cell will kill itself preventing further tumour growth

35
Q

How to use an optical microscope

A

Clip the slide onto the stage
Use the coarse adjustment knob to bring the stage up to below the objective lens
Look down the eyepiece (which contains the ocular lens)
Use the coarse adjustment knob to move the stage downwards until the image is roughly in focus
Adjust focus with fine adjustment knob
If greater magnification is needed swap to a higher powered objective lens

36
Q

Calculating mitotic index

A

Mitotic index is the proportion of cells undergoing mitosis
Number of cells with visible chromosomes/total number of cells observed

37
Q

Using a graticule and micrometer

A

An eyepiece graticule is fitted into the eyepiece
Place stage micrometer onto the stage
Line the eyepiece graticule up with the stage micrometer - each division is 0.1mm long
Divide division on stage micrometer with eyepiece graticule

38
Q

What is a specialised cell?

A

A cell that carries out a particular function