Cell Structure (4) Flashcards
cell theory
- all living organisms are composed of one or more cells
- cells are the smallest units of life
- new cells come from pre-existing cells by cell division
magnification
ratio between size of an image produced by a microscope and its actual size
resolution
ability to observe two adjacent objects as distinct from one another (measure of CLARITY)
contrast
how different one structure looks from another
light microscope
uses light for illumination
electron microscope
uses a beam of electrons for illumination
transmission electron microscopy (TEM)
thin slices stained with heavy metals
scanning electron microscopy (SEM)
sample coated with heavy metal
types of light microscopy
standard
differential interference contrast
phase contrast
fluorescence microscopy
standard (bright field)
light is focused with glass lenses
light passes directly through sample
phase contrast
microscope amplifies differences in phase of light transmitted or reflected by sample
differential interference contrast
another method using optics to improve contrast
fluorescence microscopy
uses fluorescently labeled substances
useful for visualizing specific structures
confocal microscopy
uses lasers focused at different depths of the sample to take optical sections of a sample
generates dozens of single layers of a thick specimen that can be stacked together to form very sharp image
almost always stained with fluorescent staining
prokaryotic
no nucleus
“before nucleus”
smaller than eukaryotic
eukaryotic
possess a nucleus and other membrane-bound organelle
2 kinds of prokaryotic organisms
bateria
archaea
bacteria
abundant
vast majority not harmful
smal – 1mum to 10 mum in diameter
archaea
small – 1mum to 10 mum in diameter
rare
extreme environments