Calibration methods (Lecture 21)

Question 1

How do you calculate ppm and ppb?

Answer 1

ppm = mass of substance/mass of sample x 10⁶
ppb = mass of substance/mass of sample x 10⁹

Question 2

What are the equations associated with Internal standard calibration curves?

Answer 2

Signal from analyte/Signal from standard = F x (conc of analyte/conc of standard)

A(x)/A(s) = F x ([X]f/[S]f)
Where A(x)/A(s) is the function to plot on y-axis and ([X]f/[S]f) is the function to plot on x-axis.