Bone Marrow and Hematopoiesis

Question 1

Hematopoiesis

Answer 1

• process of production of mature blood cells
  - erythropoiesis
  - luekopoiesis
• occurs within bone marrow (and thymus)
  - lymphocytes also produced in lymphatic tissues

Question 2

3 phases of hematopoiesis in the embryo

Answer 2

yolk-sac–> formation of blood islands within the yolk sac
hepatic–> hematopoietic centres appear in liver
bone marrow

Question 3

Hematopoietic stem cells express?

Answer 3

CD34+, CD90+, CD38-

Question 4

Sources of HSCs

Answer 4

bone marrow
peripheral blood- can increase this with small # of HSCs
umbilical cord blood

Question 5

Red vs Yellow Bone Marrow

Answer 5

Red
-within marrow cavity of long bone
-consists of - BV, sinusoids, hematopoietic cells
Yellow
-inactive in blood cell form
-red marrow replaced with adipose tissue

Question 6

Through erythropoiesis you see:

Answer 6

• progressive reduction in nucleus and cell size
• inactivation and extrustion of the nucleus
• progressive reduction in cytoplasmic organelles
• progressive hemoglobin synthesis by ribosomes

Question 7

Cells of erythropoiesis

Answer 7

rubriblast 
prorubricyte
basophilic rubricyte 
polychromatophilic rubricyte
-metarubricyte
-polychromatophil (reticulocyte in blood)

Question 8

Granulopoiesis

Answer 8

for neutrophils, eosinophilas and basophils
myelocyte
metamyelocyte
band cell

Question 9

Monocytic series

Answer 9

monoblast
promonocyte
monocyte –> macrophage in tissue

Question 10

Lymphopoiesis

Answer 10

• lymphocyte development
• express GATA-3–> T lymphocytes (enter thymus to diff)
• express Pax-5–> B lymphocyte (diff in bone marrow/avian bursa)