Biotech Lab “Pop” Quiz Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

SNP

A

single nucleotide polymorphism
(point mutation)

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2
Q

PTC

A

phenylcarbamide
- protein taste receptors coded for by gene TAS2R38
- Alleles: T= taster; t= non-taster

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3
Q

RFLP

A

Restriction Fragment Length Polymorphism
- a single base change prevents restriction enzyme from cutting: get DNA fragments of different lengths

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4
Q

Purpose Biotech I

A

Use RFLP-PCR to determine your genotype for PTC SNP for gene TAS2R38

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5
Q

Purpose Biotech III

A

Use PTC paper to determine your phenotype for ability to taste PTC

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6
Q

PCR

A

Polymerase Chain Reaction
1. Denature (94C) : disrupt H-bonds
2. Anneal (45-65C): 2 primers bind
3. Extensions (72C): Taq polymerase
- binds to DNA + primers
- adds nucleotides
(dATP, dCTP, dTTP, dGTP)

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7
Q

DNA Ladder

A

DNA of known lengths, used as a reference

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8
Q

genotype vs phenotype

A

genotype: an organisms genetic information
- (ex. homo, hetero) (AA, bb, Ab)
phenotype: set of observable physical traits
- (ex. white or purple flower)

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