Biotech Lab “Pop” Quiz

Question 1

SNP

Answer 1

single nucleotide polymorphism
(point mutation)

Question 2

PTC

Answer 2

phenylcarbamide
- protein taste receptors coded for by gene TAS2R38
- Alleles: T= taster; t= non-taster

Question 3

RFLP

Answer 3

Restriction Fragment Length Polymorphism
- a single base change prevents restriction enzyme from cutting: get DNA fragments of different lengths

Question 4

Purpose Biotech I

Answer 4

Use RFLP-PCR to determine your genotype for PTC SNP for gene TAS2R38

Question 5

Purpose Biotech III

Answer 5

Use PTC paper to determine your phenotype for ability to taste PTC

Question 6

PCR

Answer 6

Polymerase Chain Reaction
1. Denature (94C) : disrupt H-bonds
2. Anneal (45-65C): 2 primers bind
3. Extensions (72C): Taq polymerase
- binds to DNA + primers
- adds nucleotides
(dATP, dCTP, dTTP, dGTP)

Question 7

DNA Ladder

Answer 7

DNA of known lengths, used as a reference

Question 8

genotype vs phenotype

Answer 8

genotype: an organisms genetic information
- (ex. homo, hetero) (AA, bb, Ab)
phenotype: set of observable physical traits
- (ex. white or purple flower)