Bio Lab 2 Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

1

A

FALSE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

2

A

TRUE

The concentration of a solution is commonly listed as molarity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

3

A

FALSE

The positive control is a known substance that is expected to be positive when the test reagent is added. It is used to be sure the test
solution is working properly

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

4

A

FALSE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

5

A

FALSE

Since lipids are nonpolar, they dissolve in nonpolar solvents

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

6

A

TRUE

Phenolphthalein is used as a pH indicator because it turns red in basic conditions

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

7

A

FALSE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

8

A

TRUE

Recall that the Fluid Mosaic Model uses a phospholipid bilayer as its foundation. In this bilayer, the polar phospholipid heads face out, and the nonpolar phospholipid tails avoid water by remaining in the interior of
the membrane. The membrane interior is nonpolar, so nonpolar molecules move across readily

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

9

A

FALSE

Water will flow from an area of high water potential to an area of low potential

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

10

A

[A, B]
Stock solutions only have to be diluted, not made from scratch each time they are required

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

11

A

[A, D]

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

12

A

[A, B]

Carbohydrates are the main ingredient in soda and candy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

13

A

[D]

Steak is the only choice with the majority of its calories from protein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

14

A

[C]

Butter is mainly saturated fat.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

15

A

[B, C]

DNA exclusively can be detected using Dische diphenylamine and has thymine as a nitrogenous base.
Both DNA and RNA have cytosine as a
nitrogenous base. Ribose has an group at C2, while DNA has H in that position.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

16

A

A, B, C, D

The speed at which diffusion occurs is influenced by the size, polarity, solubility, and especially by the concentration gradient of molecules

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

17

A

B, C

Dialysis tubing has pores of a certain size, consequently, the size of a molecule is a determining factor in whether the molecule will be able to pass through dialysis tubing. Larger molecules may be too large to diffuse
through the tubing. A great difference between the concentration of a substance outside of the dialysis tubing compared to its concentration
inside the dialysis tubing is known as a steep concentration gradient. The steeper the concentration gradient, the faster diffusion will occur

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

18

A

[A, B, D]

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

19

A

[A, B, C, E]

The fluid mosaic model of cell membrane structure has a phospholipid bilayer as a foundational layer. Phospholipids are
arranged with their heads pointing out and their tails facing each other in the interior of the membrane. Interspersed amongst these phospholipids are integral proteins, which go all the way through the bilayer. Peripheral proteins occur along the side of the membrane, but do not penetrate the phospholipid bilayer. Carbohydrates extend in chains from the outside of the cell
membrane

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

20

A

A, B, C, D

All of these would be possible if human tissue could maintain viability after freezing. All of these would be possible if human tissue could maintain viability after freezing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

21

A

B

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

22

A

B

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

23

A

A

By definition, the number of grams per 100 ml refers directly to the percent of solute in that solution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

24

A

E

There are always 12 items in a dozen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

25

A

B

The number of molecules in a mole is
similar to the idea of a dozen. One dozen elephants indicates 12 individuals, and one dozen mice indicates 12 individuals. It does not matter that elephants are larger than mice; the concept relates the number of individuals only, which is 12 per dozen. One mole of a substance always indicates molecules. One mole of water weighs 18g, while one mole of glucose weighs 180g. One mole of water has the same number of molecules as does a mole of glucose, because the number of molecules per mole does not change. A mole indicates the number of individual molecules present, just as a dozen does

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

26

A

B

One atom of Mg weighs 24
One atom of S weighs 32
One atom of O weighs 16
Weight of = 24 + 32 + 4(16)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

27

A

C

The molecular weight of is 120
g/mole. 120 g/mole (0.5 moles/L) = 60 g/L

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

28

A

D

A stock solution is a concentrate that can be diluted when needed.

29
Q

29

A

C

The molecular weight of glucose is 180 g. This comes from 6 carbon atoms with atomic weights of 12 each, 12 hydrogen atoms with atomic weights of 1 each, and 6 oxygen atoms with atomic weights of 16 each.

30
Q

30

A

D

162 g / 180 grams per mole = 0.90M

31
Q

31

A

C

The most efficient antacid works by
absorbing more stomach acid

32
Q

32

A

B

Bases take up , so a buffer would
counteract this by donating

33
Q

33

A

A

Bicarbonate is a buffer of human blood and other solutions

34
Q

34

A

B

Strong acids have high concentrations. A buffer would neutralize this by picking up H+

35
Q

35

A

D

<p>Each increment of the pH scale
corresponds to a power of 10. A difference of 2 pH units is a difference of . A solution with a pH value of 4 is a weaker acid, so it would be , or 100 times weaker than a solution with a pH value of 2
</p>

36
Q

36

A

E

37
Q

37

A

A

38
Q

38

A

B

39
Q

39

A

B

The only positive test results in the example above are the Biuret test. A violet result indicates peptide bond, and therefore protein is present

40
Q

40

A

A

Lipids have many C-H bonds and little
oxygen in their fatty acid components

41
Q

41

A

A

Carbohydrates are characterized by a 1:2:1 ratio of C:H:O

42
Q

42

A

D

43
Q

43

A

C

Iodine turns blue-black in the presence of starch

44
Q

44

A

D

Diffusion is the movement of material from. high concentration to low concentration. Osmosis is a specific type of diffusion. Osmosis specifically refers to the flow of a
solvent (usually water) from high to low concentration

45
Q

45

A

B

Tonicity refers to solute concentration, but water concentration can be inferred from
tonicity. The cell is hypertonic because it has more solute (20%) than the solution it is in (5%)

46
Q

46

A

B

Since the solution in the beaker is 95% water, and the solution in the cell is 80% water, water will flow into the cell and the cell will swell

47
Q

47

A

C

Water is the solvent in this example, and osmosis is the movement of a solvent from high to low concentration.

48
Q

48

A

B

For our purposes in a lab, we can visualize the solute and the water in a beaker make up 100% of what is contained in the beaker. If we increase the amount of solute, the
proportion of water would decrease. Solute concentration and water potential are inversely proportional. As solute increases, water potential decreases

49
Q

49

A

A

Water flows from high water potential to low water potential.

50
Q

50

A

A

Tonicity refers to solute concentration, but water concentration can be inferred from
tonicity. Solutes will not move through these membranes, but water will move through and will cause a change in the appearance of the cell. In a hypertonic environment, there
is more solute and less water. Water will move from a higher concentration inside of the cell to a lower concentration outside of the cell, causing the cell to shrink

51
Q

51

A

B

As molecular weight increases, the rate of diffusion decreases. Since substance #4 has the lowest molecular weight, it will move faster than the other substances

52
Q

52

A

C

The 20% NaCl solution is hypertonic to the cell, so the cell will shrink. The cell wall is not affected by the salt solution, so it will remain in place while the cell shrinks within it.

53
Q

53

A

A

Heat is the cause of the random pattern of the Brownian movement

54
Q

54

A

A

Tonicity refers to solute concentration, but water concentration can be inferred from
tonicity. Hypertonic solutions cause cells to shrink because there is proportionally less water (and more solute) present in the environment than there is inside the cell. Through osmosis, the water would move
from high to low concentration. Due to the cell wall, a plant cell would shrink, but a decrease in size would occur inside of the strong cell wall

55
Q

55

A

A

Diffusion is the movement of material from high to low concentration. This will occur until a uniform distribution of the material is
present

56
Q

56

A

C

57
Q

57

A

C

Amphipathic refers to the possession of hydrophobic and hydrophilic regions, as exemplified by integral proteins

58
Q

58

A

C

Betacyanin, a red pigment, is housed in the central vacuole of beet cells. If this pigment is leaking, it means the cell membrane has been stressed to allow its release

59
Q

59

A

A

Recall that the composition of a cell is
mainly water, so what happens to the water will have the greatest impact in this instance. When water freezes, it expands. Inside a cell, water expansion due to freezing would rupture the cell membrane, which if
widespread would destroy tissue and cause frostbite

60
Q

60

A

D

Since methanol and acetone are nonpolar, they would act on the nonpolar fatty acid tails of the phospholipids

61
Q

61

A

A

Small, nonpolar molecules tend to pass rapidly through the cell membrane because of the nonpolar nature of the membrane interior. A polar molecule would be repelled by the nonpolar interior

62
Q

62

A

“Distilled Water”

The negative control is not expected to react with the test reagent. If it does have a positive reaction, there is contamination or a problem with the test reagent.

63
Q

63

A

“Peptide”

Peptide bonds form between amino acids in a protein

64
Q

64

A

“Permeable”

Permeability refers to the possibility that materials will move through a membrane. If a membrane allows some materials through but not others, it is called semi-permeable, or differentially permeable.

65
Q

65

A

“Dialysis”

Just as the cell membrane is differentially permeable, dialysis tubing is differentially permeable. That shared property makes dialysis tubing a good model for a cell membrane

66
Q

66

A

“Kinetic”

Kinetic energy is the energy of movement.

67
Q

67

A

“Tonoplast”

The tonoplast is a membrane that surrounds the central vacuole. The tonoplast provides channels through which materials can pass

68
Q

68

A

“Absorbance”

An increase in pigment is read by the
spectrophotometer as increased absorbance.

69
Q

69

A

“Nonpolar”

Both acetone and methanol are nonpolar.