Basic Biochem Kaplan 1 Flashcards
What enzyme is deficient in albinism? What does this enzyme normally do?
Tyrosinase, which converts Tyr to melanin.
What amino acids build up in Maple Syrup Urine disease?
branched, neutral aa’s - Leu, Ile, and Val
What important products are made from tryptophan?
5-HT, Melatonin, Niacin
Which aa is the precursor to NO?
Arg
What drugs block glutamate receptors?
Felbamate and Ketamine
What 2 types of post-translational modification occur at Ser and Thr residues?
O-glycosylation & O-phosphorylation
3 uses of N-acetylcysteine
- paracetamol poisoning 2. break up mucus plugs in CF 3. treat hemorrhagic cystitis from cyclophosphamide
What drug prophylaxes against bladder toxicity from cyclophosphamide?
MESNA
What amino acid provides the methyl groups necessary to make the 7-methylguanosine 5’ cap and convert NE to Epi, along w/ many other rxns?
Methionine in the form of SAM
What 2 aa’s undergo N-glycosylation?
Gln & Asn
Label the bands on the electrophosesis gel for the correct type of hemoglobin: HbS, HbC, or HbA.
Which amino acid is only essential during growth?
Arg
Michaelis-Menten equation
V = (k2[E] [S]) / (Km + [S])
or
V = (Vmax[S]) / (Km + [S])
Define Km
the substrate concentration at which rate is 1/2 Vmax
What is the y-axis of the Lineweaver-Burk plot?
1/V
What kind of enzyme inhibition is this?
non-competitive
What kind of enzyme inhibition is this?
competitive
