Bacteria 1 Flashcards
What’s the bacteria called?
Staphylococcus aureus
What oral diseases are caused by this bacteria
Angular cheilitis, mucositis, and bone
What are the routes called
Endogenous (from within one’s own body) and exogenous (cross infection)
Describe the routes
How is the disease called where the angles of mouth are red and crusty
Angular cheilitis
How is the disease sampled
Sample sides of angles with sterile swab and moistened with sterile water.
What is the laboratory test
Gram stain
Identification rapid benchside
Growth on selective agar (mannitol salt agar) and non selective (blood agar), incubated, colony morphology, gram stain, clumping factor detection (coagulate positive virulence factor)
Selective agar example
Salt preserves, S. aureus ferments mannitol decreases pH which is detected by pH meter. S. Epidermis does not cause a change
Colony morphology
Round, convex and 1-4mm in diameter with sharp border
What’s the other test
Coagulate (clumping factor) test
Describe the second test
S. Aureus caused coagulation/clumping of serum and is distinct. Test tube filled with serum, inocculum added, waited several hours, solid clot formed. This is how it also caused a clinical infection (pus) caused by coagulase virulence factor
Clumping factor detection new method
Slide agglutination test.
Describe the new method
Clumping factor and protein A found on S. aureus cell wall. Fibrinogen and IgG to protein A is on the surface of the Staphaurex latex beads. S aureus will clump in the Staphaurex latex bead solution and positively identifies S. aureus
How to name bacteria
Genus (clan) then Species
