9.4 Sequencing genomes Flashcards
1
Q
The Human Genome project began using the ?
A
hierarchical strategy
2
Q
What are the 5 steps of the hierarchical strategy?
A
- Construct a BAC genomic library
- Identify sets of overlapping BAC clones
- Shear DBA from each BAC separately to make smaller clones
- Sequence DNA
- Assemble sequences based on overlap
3
Q
What is the hierarchical strategy(hierarchical shotgun sequencing)?
A
It is a method used to sequence large genomes such as the human genome. his approach involves breaking down the genome into smaller, manageable pieces, sequencing them, and then assembling the complete sequence.
4
Q
Who developed the shotgun strategy and why?
A
Celera did in order to sequence the whole-genome
5
Q
What are the three steps of shotgun strategy
A
- Shear genomic DNA to make shotgun (uncharacterized) clones
- Sequence DNA
- Assemble sequences based on overlap
6
Q
The whole-genome shotgun approach can be highly __________
A
automated
7
Q
The shotgun strategy helps overcome problems due to repetitive ____
A
DNA
