[41] Molecular Biology Techniques Flashcards
What is the focus of “Molecular Biology Techniques”?
The focus is on methods used to study the molecular basis of biological activity.
What is molecular biology?
It is a field of biology that studies the composition, structure and interactions of cellular molecules such as DNA, RNA, and proteins.
Can you name a few common molecular biology techniques?
- Polymerase Chain Reaction (PCR)
- Gel Electrophoresis
- Cloning
- Southern Blotting
- DNA Sequencing
What is the Polymerase Chain Reaction (PCR)?
It’s a method used to amplify a specific DNA segment.
What is the purpose of PCR in molecular biology?
It helps to produce enough DNA for further analysis or manipulation.
What is Gel Electrophoresis?
It’s a technique for separating DNA, RNA, or proteins based on size and charge.
What is the purpose of Gel Electrophoresis in molecular biology?
It allows for the visualization and analysis of DNA, RNA, or proteins.
What does Cloning mean in molecular biology?
It refers to the process of producing identical copies of a DNA sequence.
What is the purpose of Cloning in molecular biology?
It enables the replication of specific genes or DNA sequences for further study.
What is Southern Blotting?
It’s a method used to detect a specific DNA sequence in a DNA sample.
What is the purpose of Southern Blotting in molecular biology?
It allows for the identification and characterization of specific DNA sequences.
What is DNA Sequencing?
It is the process of determining the exact order of the nucleotides in a DNA molecule.
What is the purpose of DNA Sequencing in molecular biology?
It helps to determine the precise genetic information contained in a DNA molecule.
What is Restriction Digest?
It’s a process where DNA is cut at specific sites using restriction enzymes.
What is the purpose of Restriction Digest in molecular biology?
It’s used for manipulating DNA for cloning or analysis.