[41] Molecular Biology Techniques Flashcards

1
Q

What is the focus of “Molecular Biology Techniques”?

A

The focus is on methods used to study the molecular basis of biological activity.

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2
Q

What is molecular biology?

A

It is a field of biology that studies the composition, structure and interactions of cellular molecules such as DNA, RNA, and proteins.

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3
Q

Can you name a few common molecular biology techniques?

A
  1. Polymerase Chain Reaction (PCR)
  2. Gel Electrophoresis
  3. Cloning
  4. Southern Blotting
  5. DNA Sequencing
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4
Q

What is the Polymerase Chain Reaction (PCR)?

A

It’s a method used to amplify a specific DNA segment.

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5
Q

What is the purpose of PCR in molecular biology?

A

It helps to produce enough DNA for further analysis or manipulation.

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6
Q

What is Gel Electrophoresis?

A

It’s a technique for separating DNA, RNA, or proteins based on size and charge.

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7
Q

What is the purpose of Gel Electrophoresis in molecular biology?

A

It allows for the visualization and analysis of DNA, RNA, or proteins.

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8
Q

What does Cloning mean in molecular biology?

A

It refers to the process of producing identical copies of a DNA sequence.

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9
Q

What is the purpose of Cloning in molecular biology?

A

It enables the replication of specific genes or DNA sequences for further study.

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10
Q

What is Southern Blotting?

A

It’s a method used to detect a specific DNA sequence in a DNA sample.

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11
Q

What is the purpose of Southern Blotting in molecular biology?

A

It allows for the identification and characterization of specific DNA sequences.

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12
Q

What is DNA Sequencing?

A

It is the process of determining the exact order of the nucleotides in a DNA molecule.

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13
Q

What is the purpose of DNA Sequencing in molecular biology?

A

It helps to determine the precise genetic information contained in a DNA molecule.

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14
Q

What is Restriction Digest?

A

It’s a process where DNA is cut at specific sites using restriction enzymes.

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15
Q

What is the purpose of Restriction Digest in molecular biology?

A

It’s used for manipulating DNA for cloning or analysis.

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16
Q

What is cDNA Library?

A

It’s a collection of DNA sequences that collectively express the functional elements of a genome.

17
Q

What is the purpose of a cDNA Library in molecular biology?

A

It allows for the study of gene expression in a particular tissue at a particular time.

18
Q

What is a Microarray?

A

It’s a high-throughput technology used to detect and measure the expression levels of thousands of genes simultaneously.

19
Q

What is the purpose of a Microarray in molecular biology?

A

It provides insight into gene expression patterns in different tissues or at different times.

20
Q

What is Northern Blotting?

A

It’s a technique used to detect specific RNA molecules within a mixture.

21
Q

What is the purpose of Northern Blotting in molecular biology?

A

It helps in studying gene expression by detecting specific RNA molecules.