300 Questions Flashcards

1
Q

To examine morphology of bacteria, which stain should be used?

A

Methylene blue stain

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2
Q

A cultural medium used to grow ringworm is

A

Sabourauds agar

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3
Q

The mordant in a gram stain is

A

Lugols iodine

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4
Q

Gram negative bacteria stain is

A

Red

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5
Q

Gram positive bacteria stain is

A

Blue

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6
Q

Stain used to detect acid fast bacteria is

A

Ziehl-neelson stain

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7
Q

Which medium is used for growing salmonellae

A

Deoxycholate citrate agar

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8
Q

Drug group of choice for feline pneumonitis and fie are

A

Tetracyclines

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9
Q

When posting individual pathological sample, size of container must not exceed

A

50ml

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10
Q

When preserving tissue samples for toxicology, best preservative is

A

95% ethanol

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11
Q

Containers for despatch of tissue samples should be

A

Wide necked

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12
Q

Autolysis is

A

Digestion of tissue by its own enzymes

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13
Q

What’s a thoma pipette used for

A

Rbc and wbc counts

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14
Q

What’s leishmans stain used for

A

To stain blood smear for identifying wbcs

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15
Q

What may cause neutrophilia?

A

Acute blood infection

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16
Q

Triple phosphate crystals most commonly formed in

A

Alkaline urine

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17
Q

You would microscopically identify starch granules in a Faecal sample

A

As black dots

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18
Q

Most common urine crystal in cats is

A

Struvite crystals

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19
Q

A spectrophotometer used to

A

Measure enzyme levels in blood

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20
Q

Disintegration caused by blood being stored at too high a temperature is called

A

Desiccation

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21
Q

When preserving pathological specimens, the vol of formalin should

A

Exceed ten times formalin to tissue

22
Q

Which preservative is particularly toxic to humans

A

Toluene

23
Q

Acth stim test

A

Basal cortisol level is first measured
Then patient is given an iv injection of synthetic Acth which will stimulate release of cortisol
A second sample is taken two hours later
Elevated levels of cortisol indicate cushings
Decreased levels indicate Addison

24
Q

Low dose dexmethasone suppression test

A

Basal cortisol level is measured
Then given iv low dose dex
Second sample is take 8 hrs later
Cortisol levels should normally decrease
A high post Injection cortisol level indicates hyperadrenocorticism
An overnight dex suppression test is performed in horses with suspected cushings

25
Q

High dose dex suppression test

A

Used to distinguish between pituitary dependant and non pituitary dependent hyperadrenocorticism in dogs
Basal cortisol level measured
Then high dose dex given iv.
Sample taken three hours later and a third 8 hours
Patients with pituitary dependent cushings commonly have low level
Non pituitary dependent will have above base level

26
Q

Normal sg

A

Dog

1.015-1.045

27
Q

Normal sg cat

A

1.035-1.060

28
Q

Horses normal sg

A

1.020-1.050

29
Q

Normal urine pH cat

A

7-9

30
Q

Normal u r ine pH dog

A

5-7

31
Q

Normal pH horse urine

A

7-9

32
Q

Urine protein increase

A

Rf
Hge
Inflammatory disease

33
Q

Urine glucose increase

A

Stress, excitement, diabetes mellitus, cushings, hyperthyroidism

34
Q

Nitrate in urine

A

Bacterial infection

35
Q

Hyaline casts

A

Clear, colourless, composed of protein
Present in patients with poor renal perfusion
Fever
Strenuous exercise

36
Q

Granular casts

A

Most common cast in animal urine
Appear granular due to degeneration of other cells such as rbcs
Seen where acute nephritis and chronic renal failure

37
Q

Epithelial casts

A

Epithelial cells from renal tubules in a hyaline matrix

Seen in patients with acute nephritis

38
Q

Leucocyte casts

A

Contains white blood cells-normally neutrophils

Inflammation in renal tubules

39
Q

Erythrocyte casts

A

Indicate renal haemorrhage

40
Q

Fatty casts

A

Cats with renal disease

Dogs with dm

41
Q

Cellular casts are rarely seen in

A

Equine urine, hyaline casts ocassionallyy seen

42
Q

Struvite (magnesium phosphate or triple phosphate) crystals

A

Found in alkaline urine

43
Q

Cysteine crystals

A

Found in acidic urine
Renal tubular dysfunction
Not seen in equine

44
Q

Calcium oxalate

A

Dihydrate appear as small square crystals with x in centre
Found in acidic or neutral urine
Some breeds predisposed (yorkie, mini poodle, Persian cats, mini schnauzer)
Ethylene glycol poisoning
Rarely seen in equine

45
Q

Ammonium urate crystals

A

Spindles, thorn apple shaped or rosettes
Found in acidic or neutral urine
Clinically normal in Dalmatians due to way they metabolite and excrete protein
In other dogs and cats can mean liver disease or portosystemic shunt
Not seen in equine

46
Q

Uric acid crystals

A

Vary in shape
Diamond or rhomboid shape
Acidic urine
Rare

47
Q

Calcium phosphate crystals

A

Long and flat with rectangular outline
Alkaline urine
Rare

48
Q

Calcium carbobate crystals

A

Normal in equine and rabbit

Causes cloudy or turbid urine

49
Q

Equine faeces

A

Large amount of cellulose plus undigested seeds, water and electrolytes

50
Q

Modified mcmaster technique

A

Number of eggs per gram of faeces=

(eggs in grid 1 + eggs in grid 2) x50