23: staphylococci vignette - sheet 1 - jonathan

Question 1

Explain the gram stain and colony morphology of
S. aureus
S. epidermidis
S. saprophyticus

Answer 1

In staphylococci, which species has
G+ cocci in CLUSTERS, YELLOW colony
G+ cocci in CLUSTERS, WHITE colony
G+ cocci in CLUSTERS, color not specified

Question 2

What are the catalase vs coagulase status of
S. aureus
S. epidermidis
S. saprophyticus

Answer 2

Which staph are
Catalase + and Coagulase +
Catalase + and Coagulase -
Catalase + and Coagulase -

Question 3

Which have resistance to NOVOBIOCIN?
S. aureus
S. epidermidis
S. saprophyticus

Answer 3

sensitive
sensitive
RESISTANT

Question 4

Which have hemolysis
S. aureus
S. epidermidis
S. saprophyticus

Answer 4

S. aureus has hemolysis (alpha toxin)

Question 5

Which have surface protein A
S. aureus
S. epidermidis
S. saprophyticus

Answer 5

S. aureus has surface protein A

Question 6

In what tissues do each reside?
S. aureus
S. epidermidis
S. saprophyticus

Answer 6

What staph reside…
Skin and nares
Skin and mucus membranes
Skin and GI tract

Question 7

What are the clincal syndromes of
S. aureus
S. epidermidis
S. saprophyticus

Answer 7

1) toxin-mediated disease (food poisoning, TSS, and SSSS) and Invasive syndromes
2) Invasive syndrome commonly associated with foreign bodies
3) UTIs in young females attaches to uroepithelium via hemagluttanin

Question 8

What is Tx for
S. aureus
S. epidermidis
S. saprophyticus

Answer 8

1) MSSA - beta lactams and/or MRSA -vancomycin, daptomycin, linezolid, ceftaroline
2) Vancomycin (reistant to beta-lactams)
3) Bactrim

Question 9

What is Px for
S. aureus
S. epidermidis
S. saprophyticus

Answer 9

1) Handwashing and isolation
2) Handwashing
3) Not listed