2.3 Enzyme Kinetics

Question 1

michaelis menten plot

Answer 1

Question 2

Km

Answer 2

substrate concentration at half vmax

Question 3

high km =

Answer 3

low affinity of enzyme for substrate

Question 4

low km =

Answer 4

high affinity of enzyme for substrate

Question 5

vmax

Answer 5

the maximum reaction rate for a given enzyme concentration

all enzyme are saturated with subtrate

Question 6

michaelis-menten equation

Answer 6

Question 7

V0

Answer 7

initial velocity

Question 8

cooperative enzymes

Answer 8

Enzymes with multiple subunits and multiple active sites

enzymes that result in a sigmoidal michaelis-menten plot rather than hyberbolic

Question 9

positively cooperative binding

Answer 9

after one ligand is bound, the affinity of the enzyme for further ligands increases

Question 10

negatively cooperative binding

Answer 10

after one ligand is bound, the affinity of the enzyme for further ligands decreases

Question 11

what numerical value represents cooperativity

Answer 11

Hill’s Coefficient

Question 12

Kcat

Answer 12

the number of substrate molecules converted to products per second

Question 13

large Kcat value means…

Answer 13

the enzyme is converting a high number of substrate very quickly

Question 14

small Kcat value means…

Answer 14

the enzyme is slowly converting substrate to product

Question 15

an efficient enzyme will have a — kcat value and a — km value

Answer 15

an efficient enzyme will have a high kcat value and a small km value

Question 16

the [smaller/larger] the kcat/km value, the more efficient the enzyme

Answer 16

the larger the kcat/km value, the more efficient the enzyme