1.3 Microscopy Flashcards
1
Q
What is the function of the eye piece lens and objective lens?
A
- Magnify the object/ make the object look bigger
2
Q
What is the function of the coarse focus and fine focus?
A
- Increase the clarity of the image/ make the image clearer
3
Q
How do you calculate total magnification?
A
total magnification = eye piece lens X objective lens
4
Q
What is magnification?
A
How much bigger the image is compared to the actual specimen
5
Q
What is resolution?
A
The smallest distance between two points that can be seen as two points
6
Q
How do you calculate image size?
A
image size = magnification X actual size of specimen
7
Q
What is field of view?
A
The circular area you see in the light microscope
8
Q
How do you calculate estimated size?
A
field of view (in mm) ÷ number of cells you can see across the field of view
9
Q
Factors of a light microscope
A
- Works with light reflecting of an image
- Cheaper and more accessible than the electron microscope
- Allows animal, plant and bacteria cells to be compared and viewed
10
Q
Factors of an electron microscope
A
- Works with electrons reflecting of an image
- Higher magnification (can see smaller objects)
- Higher resolution (can see more detail)
- Higher resolution means we can investigate more about the function of structures inside the cells (e.g. the way mitochondria provides energy for cells)
