1.1c Flashcards
What is a centrifuge used for?
to allow substances to separated according to their density
More dense components do what?
settle in a pellet
Less dense components do what?
remain in the supernatant
Paper and thin layer chromatography can be used for…
separating substances such as amino acids and sugars
The speed that each solute travels along the chromatogram depends on…
its differing solubility in the solvent used
Affinity chromatography relies on…
the binding interaction between a protein and another substance (ligand)
A solid matrix or gel column is created with…
specific molecules bound to the matrix or gel
Soluble, target proteins in a mixture, with high affinity for these molecules become…
attached to them as the mixture passes down the column
Other non-target molecules with weaker affinity are…
washed out
Gel electrophoresis uses…
currents flowing through a buffer to separate proteins and nucleic acids
