1.1 Microscopy Flashcards
What were the first type of microscope?
Light
What are the two types of lense in a compound light microscope?
Objective and eyepiece
What are the parts of a microscope?
Eyepiece lense Turret objective lense coarse focusing knob fine focusing knob specimen stage
What types of mount are there?
Dry - cover slip placed over slice of specimen
Wet - specimen in liquid and cover slip placed on from angle
Define Magnification
how many time larger the image is than the actual object
Define Resolution
ability to see individual objects as separate objects
What limits resolution? and how it can be resolved?
It is limited by diffraction of light
Use bean of electrons which have wavelength thousands time shorter than light
Calculation for magnification
mag = size of image / actual size
How man micrometers (um) are in a millimetre (mm)
1000
how do you get from nanometers to micrometers
divide by 1000
300nm = 0.3 um
What are the 3 measurements in microscopy?
Nanometres nm
Micrometers um
Millimetres mm
What is the difference between magnification and resolution?
Mag is how much bigger image is then original image
Resolution is ability to distinguish between two points
Whats resolution of light microscope
50-200nm
What is resolution of transmission electron microscope
0.05-1.0nm
What is benefit of using scanning electron microscope?
Produced 3D image with surface detail
