10. Engineering Contractile Tissues for Hearth Repair Flashcards
Does heart have limited repair capacity?
Yes Lol just fking kidding
FFS
Apparently heart is self renewing organ due to stem cells
Troll lecture is troll
What is cardiomyocyte cell death replaced by?
Scar tissue
What happens in response to increased load by left over cells that arent dead?
Hypertrophy - They need to work harder cos ther others tapped out
What stem cells repair heart?
Adult stem cells Found in clusters/niches Rare
What is a cell marker expressed by adult stem cells in heart?
GATA-4
What are some issues with useing adult stem cells for treating heart failure?
Cell source Delivery / mobilization Recruitment / Homing chemokines Retention / adhesion Differentiation - unknown mechanisms Non-differentiation effects
What happens to patients surviving myocardial infarction?
Progressive fibrosis and dilatation Need heart transplant
What are the major elements of tissue engineering in vivo?
1) Cells
2) Scaffold
3) Growth Factors
4) Vascularisation
What were 3 attempts at in vitro cardiac tissue engineering?
1) Mechanically stretched ring-shaped engineered heart tissue
2) Stacking of cardiomyocytes monolayer (cell sheet)
3) Seeding of cardiomyocytes on decellularized rat heart
What is preconditioning?
Giving celsl period of hypoxic stress After implantation, cells survive much better
What is in vivo preconditioning?
Make animal leg hypoxic Implant tissue
What cardiac engineering methods are there and what are their limitations and benefits?
What are some potential cardiomyocytes sources?
Proliferative therapies
Stemm cell therapies
- ESC
- iPSC
Direct reprogramming
How is fat used to repair heart (theoretically)?
Liposuction
Stem celsl isolated
Grown in lab
Treated to become muscle cells
Grown into heart tissue
Applied to heart
What turned adipose derived stem cells into cardiac muscle cells?
Direct co-culture AS and cardiac muscle cells
Paracrine activity
